Once glucose is converted into glucose 6-phosphate, it is then isomerized into fructose 6-phosphate. The basic reason behind this conversion is that the addition of one more phosphate into glucose is not possible, due to its cyclic structure. Where only one carbon atom is present outside of the ring unlike fructose where two carbon atoms are present on the outside of the ring. And hence, two phosphates can attach to a fructose ring.
Therefore, glucose-6-phosphate is first isomerized to fructose-6-phosphate for further phosphorylation under the category of hexose-phosphate isomerase.
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