The 3-phosphoglyceric acid hence obtained is now mutated to form 2-phosphoglyceric acid by the activity of enzyme mutase.
The position of phosphate is changed from 3rd carbon to 2nd carbon for the formation of Phosphoenolpyruvate.
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The 2-phosphoglyceric acid hence obtained is now dehydrated to form phosphoenolpyruvate acid. This activity is under the control of enzyme enolase and result in the removal of a water molecule from the substrate.
The 1,3-BPG hence obtained from the partial oxidation of G3P undergoes substrate-level phosphorylation. An inorganic phosphate is released by the substrate named 1,3-BPG which is taken by an ADP molecule and hence results in the formation of an ATP molecule.
This 1,3-BPG gets now converted...
The phosphoenolpyruvate obtained is now under the activity of enzyme pyruvatekinase and is converted into pyruvic acid along with the formation of an ATP as a premium-product.
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